Coding

Part:BBa_K2460004:Design

Designed by: Haiyun Liu   Group: iGEM17_Fudan_China   (2017-10-21)


φRv1 integrase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 274
    Illegal NgoMIV site found at 391
    Illegal NgoMIV site found at 422
    Illegal NgoMIV site found at 958
    Illegal AgeI site found at 1364
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • Note that if you are using a repressor to regulate the integrase, make sure there are already repressors accumulated in the cell to prevent integrase from expressing.
  • An attB/P site follwing after a promoter may reduce experssion.
  • This part has a 6×His tag and a thrombin site, and mutated to be RFC10 compatible.


Source

Lab storage

References